Model Number: Dengue NS1 Brand Name: EGENS Key Specifications/Special Features: Dengueantigentestcassette(wholeblood)Specimencollection:Forwholebloodspecimen,collectfreshbloodspecimenjustpriortousingtheassaySpecimensmustbefreshWholebloodcollectedbyvenipunctureshouldbestoredat2 to 8°Cifthetestistoberunwithin2daysofcollection

Donotfreezewholebloodspecimens

Wholebloodcollectedbyfingerstickshouldbetestedimmediately

Testprocedure:

• Step1:bringthespecimenandtestcomponentstoroomtemperatureifrefrigeratedorfrozen, mixthespecimen wellpriorto assayonce thawed
• Step2:whenreadytotest,openthepouchatthenotchandremovedevice,placethetestdeviceonaclean,flatsurface
• Step3:besuretolabelthedevicewithspecimen’s IDnumber
• Step4:fillthepipettewiththespecimen, holdingthedropper vertically (referenceasthebelowpicture),dispense1drop(about 5L)ofspecimenintothesamplewellmakingsurethattherearenoairbubbles
• Dilutethespecimen10timeswithdiluents (e.g.pipette 1dropabout5lof specimenintothe samewellandthenadd 1drop about 50lofdiluentsintothesamewell)
• Step5:setuptimer
• Step6:resultscanbereadwithin15minutes,positiveresultscanbevisibleinasshortas1minute,don notreadresultafter 20minutes, to avoid confusion,discardthetestdeviceafterinterpretingtheresult

Negative:onlythecontrollineisnegative

Positive:boththecontrollineandtestlineappear

Invalid:controllineabsentregardlessofotherlines indicates, testis invalidandmustberepeated